研究業績

    *:Corresponding author

    2023年

  1. Imaizumi T, Hayashi R*, Kudo Y, Li X, Yamaguchi K, Shibata S, Okubo T, Ishii T, Honma Y, Nishida K. Ocular instillation of conditioned medium from mesenchymal stem cells is effective for dry eye syndrome by improving corneal barrier function. Sci Rep. 2023 Aug 11;13(1):13100.
  2. Kitao M, Hayashi R*, Nomi K, Kobayashi R, Katayama T, Takayanagi H, Oguchi A, Murakawa Y, Nishida K. Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker. iScience 2023 Jun 5;26(7):107016.

    3. 2022年

  3. Yoshinaga Y, Soma T, Azuma S, Maruyama K, Hashikawa Y, Katayama T, Sasamoto Y, Takayanagi H, Hosen N, Shiina T, Ogasawara K, Hayashi R, Nishida K. Long-term survival in non-human primates of stem cell-derived, MHC-unmatched corneal epithelial cell sheets. Stem Cell Reports. 2022 Jul 12;17(7):1714-1729.
  4. Hayashi R*, Okubo T, Kudo Y, Ishikawa Y, Imaizumi T, Suzuki K, Shibata S, Katayama T, Park SJ, Young RD, Quantock AJ, Nishida K. Generation of 3D lacrimal　gland organoids from human pluripotent stem cells. Nature 2022　May;605(7908):126-131. doi: 10.1038/s41586-022-04613-4.
  5. Toru Okubo, Hayashi R*, Yuji Kudo, Ishikawa Y, and Quantock A. Fabrication of three-dimensional lacrimal gland-like tissue organoids from human pluripotent stem cells. Procol exchange 2022, doi: 10.21203/rs.3.pex-1821/v1

    6. 2021年

  6. Shiraki N, Maruyama K, Hayashi R*, Oguchi A, Murakawa Y, Katayama T, Takigawa T, Sakimoto S, Quantock AJ, Tsujikawa M, Nishida K. PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids. Stem Cell Reports. 2022 Feb 8;17(2):221-230.
  7. Watanabe S, Hayashi R*, Sasamoto Y, Tsujikawa M, Ksander BR, Frank MH,　Quantock AJ, Frank NY, Nishida K. Human iPS cells engender corneal epithelial　stem cells with holoclone-forming capabilities. iScience 2021 Jun 5;24(6):102688. doi: 10.1016/j.isci.2021.102688.
  8. Nomi K, Hayashi R*, Ishikawa Y, Kobayashi Y, Katayama T, Quantock AJ, Nishida K, Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs. Cell Rep. 2021 Feb 2;34(5):108715.
  9. Ashworth S, Harrington J, Hammond G.M., Bains K.K, Koudouna E, Hayes A. J., Ralphs J.R., Regini J.W., Young R.D, Hayashi R, Nishida K., Hughes C. E, Quantock AJ, Chondroitin Sulfate as a Potential Modulator of the Stem Cell Niche in Cornea. Front. Cell Dev. Biol. 2021 Jan 12;8:567358

    10. 2020年

  10. Hashikawa Y, Hayashi R*, Tajima M, Okubo T, Azuma S, Kuwamura M, Takai N, Osada Y, Kunihiro Y, Mashimo T, Nishida K, Generation of knockout rabbits with X-linked severe combined immunodeficiency (X-SCID) using CRISPR/Cas9. Sci Rep. 2020 Jun 19;10(1):9957.
  11. Kobayashi Y, Hayashi R*, Shibata S, Quantock AJ, Nishida K, Ocular Surface Ectoderm Instigated by WNT inhibition and BMP4. Stem Cell Res. 2020 Jul;46:101868.
  12. Shibata S, Hayashi R*, Kudo Y, Okubo T, Katayama T, Ishikawa Y, Kobayashi Y, Toga J, Taniguchi Y, Honma Y, Sekiguchi K, and Nishida K, Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium. Stem Cell Rep. 2020 Apr 14;14(4):663-676.
  13. Okubo T, Hayashi R*, Shibata S, Kudo Y, Ishikawa Y, Inoue S, Kobayashi Y, Honda A, Honma Y, Kawasaki S, Nishida K, Generation and validation of a PITX2–EGFP reporter line of human induced pluripotent stem cells enables isolation of periocular mesenchymal cells. J. Biol. Chem. 2020 Mar 13;295(11):3456-3465.

    14. 2019年

  14. Fujimoto S, Hayashi R*, Hara S, Sasamoto Y, Harrington J, Tsujikawa M, and Nishida K, KLF4 prevents epithelial to mesenchymal transition in human corneal epithelial cells via endogenous TGF-β2 suppression. Regen Ther. 2019 Dec 11:11: 249-257.
  15. Shibata S, Hayashi R*, Okubo T, Kudo Y, Baba K, Honma Y, Nishida K. The secretome of adipose-derived mesenchymal stem cells attenuates epithelial-mesenchymal transition in human corneal epithelium. Regen Ther. 2019 Jul 2;11:114-122. eCollection 2019 Dec.

    16. 2018年

  16. Xu P, Kai C, Kobayashi Y, Yamamoto Y, Tsujikawa M, Hayashi R, and Nishida K. A new in vitro model of gelatinous drop-like corneal dystrophy by knocking out TACSTD2 and its paralogous gene EpCAM in human corneal epithelial cells.  Transl Vis Sci Technol. 2018 Nov; 7(6): 30.
  17. Hayashi R*, Ishikawa Y, Katori R, Takayanagi H and Nishida K. CD200 facilitates the isolation of corneal epithelial cells derived from human pluripotent stem cells. Sci Rep. 2018 Nov. 8(1):16550
  18. Shibata S, Hayashi R*, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J,  Yagi E, Honma Y, Quantock AJ, Skiguchi K and Nishida K. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages. Cell Rep. 2018 Nov 6;25(6):1668-1679.e5.
  19. Okubo T, Hayashi R*, Shibata S, Kudo Y, Honma Y, Nishida K. Use of homeobox　gene expression patterns to determine anatomical regions of origin for body　surface tissues derived from adult mice. J Tissue Eng Regen Med. 2018　Jun;12(6):1412-1419. Epub 2018 May 10

    20. 2017年

  20. Kobayashi Y, Hayashi R*, Quantock AJ, and Nishida K. Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells. Stem Cell Res. 2017 Dec;25:256-265,
  21. Uesugi K, Sakaguchi H, Hayashida Y, Hayashi R, Baba K, Suganuma Y, Yokoi H,　Tsujikawa M, Nishida K. A Self-Assembling Peptide Gel as a Vitreous Substitute: A Rabbit Study. Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4068-4075.
  22. Yoshihara M, Sasamoto Y, Hayashi R, Ishikawa Y, Tsujikawa M, Hayashizaki Y,　Itoh M, Kawaji H, Nishida K. High-resolution promoter map of human limbal　epithelial cells cultured with keratinocyte growth factor and rho kinase inhibitor. Sci Rep. 2017 Jun 6;7(1):2845.
  23. Hayashi R*, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, and Nishida K. Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nat Protoc. 2017 Apr;12(4):683-696.

    24. 2016年

  24. Katori R, Hayashi R*, Kobayashi Y, Kobayashi E, Nishida K. Ebselen Preserves Tissue-Engineered Cell Sheets and their Stem Cells in Hypothermic Conditions. Sci Rep. 2016 Dec 14;6:38987.
  25. Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock A, Tsujikawa M, & Nishida K. Co-ordinated ocular development from human iPS cells and recovery of corneal function. Nature. 2016 Mar 17;531(7594):376-80.
  26. Sasamoto Y, Hayashi R, Park S, Saito-Adachi M, Suzuki Y, Kawasaki S, Quantock A, Nakai K, Tsujikawa M, and Nishida K, PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes. Sci Rep. 2016 Feb 22;6:20807.

    27. 2015年

  27. Ono M, Suzawa Y, Takami M; YamamotoG, Hosono T, Yamada A; Suzuki D, Yoshimura K, Watahiki J, Hayashi R, Arata S, Mishima K, Nishida K, Osumi N, Maki K, Kamijo R. Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice. Biochem Biophys Res Commun. 2015 Sep 4;464(4):1209-14.
  28. Kageyama T, Hayashi R, Hara S, Yoshikawa K, Ishikawa Y, Yamato M, Nishida K. Spontaneous acquisition of infinite proliferative capacity by a rabbit corneal endothelial cell line with maintenance of phenotypic and physiological characteristics. J Tissue Eng Regen Med. 2015 Mar 11.

    29. 2014年

  29. Soma T, Hayashi R, Sugiyama H, Tsujikawa M, Kanayama S, Oie Y, Nishida K. Maintenance and Distribution of Epithelial Stem/Progenitor Cells after Corneal Reconstruction Using Oral Mucosal Epithelial Cell Sheets.  PLoS One. 2014;9(10):e110987.
  30. Hara S, Hayashi R, Soma T, Kageyama T, Duncan T, Tsujikawa M, Nishida K.Identification and potential application of human corneal endothelial progenitor cells. Stem Cells Dev. 2014 Sep 15;23(18):2190-201.
  31. Hagiwara K, Obayashi T, Sakayori N, Yamanishi E, Hayashi R, Osumi N, Nakazawa T, Nishida K. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells. PLoS One. 2014 Jan 20;9(1):e84072. doi: 10.1371/journal.pone.0084072. eCollection 2014.
  32. Oie Y, Nozaki T, Takayanagi H, Hara S, Hayashi R, Takeda S, Mori K, Moriya N, Soma T, Tsujikawa M, Saito K, Nishida K. Development of a cell sheet transportation technique for regenerative medicine. Tissue Eng Part C Methods. 2014 May;20(5):373-82.

    33. 2013年

  33. Hayashi R*, Himori N, Taguchi K, Ishikawa Y, Uesugi K, Ito M, Yamamoto M, Thomas D, Tsujikawa M, Nakazawa T, Yamamoto M, Nishida K, The Role of Nrf2-Mediated Defense System in Corneal Epithelial Wound Healing. Free Radic Biol Med. 2013 Apr12;61C:333-342.
  34. Hatou S, Higa K, Inagaki E, Yoshida S, Kimura E, Hayashi R, Tsujikawa M, Tsubota K, Nishida K, Shimmura S. Validation of Na,K-ATPase pump function of corneal endothelial cells for corneal regenerative medicine. Tissue Eng Part C Methods. 2013 Dec;19(12):901-10. doi: 10.1089/ten.TEC.2013.0030.

    35. 2012年

  35. Hayashi R, Ishikawa Y, Kageyama T, Ito M, Takashiba K, Fujioka T, Tsujikawa M, Miyoshi Y, Yamato M, Nakamura Y and Nishida K. Generation of Corneal Epithelial Cells from Induced Pluripotent Stem Cells Derived from Human Dermal Fibroblast and Limbal Epithelium. PLoS One. 2012;7(9) e45435

    36. 2011年

  36. Watanabe R, Hayashi R, Kimura Y, Tanaka Y, Kageyama T, Hara S, Tabata Y, Nishida K. A novel gelatin hydrogel carrier sheet for corneal endothelial transplantation. Tissue Eng Part A. 2011 Sep;17(17-18):2213-9.
  37. Sakurai M, Hayashi R, Kageyama T, Yamato M, Nishida K, Induction of epithelial progenitor cells in vitro from induced pluripotent stem cells. J Artif Organs. 2011 Mar;14(1):58-66. Epub 2011 Feb 5.
  38. Kikuchi M, Hayashi R, Kanakubo S, Ogasawara Y, Yamato M, Osumi N, Nishida K, Neural crest-derived multipotent cells in the adult mouse iris stroma. Genes to Cells. 2011 Mar;16(3):273-81

    39. 2010年

  39. Oie Y, Hayashi R, Takagi R, Yamato M, Takayanagi H, Tano Y, Nishida K. A novel method of culturing human oral mucosal epithelial cell sheet using post-mitotic human dermal fibroblast feeder cells and modified keratinocyte culture medium for ocular surface reconstruction. Br J Ophthalmol. 2010 94:1244-1250.
  40. Hayashi R, Yamato M, Takayanagi H, Oie Y, Kubota A, Hori Y, Okano T, Nishida K, Validation system of tissue engineered epithelial cell sheets for corneal regenerative medicine. Tissue Engineering Part C. 2010 Aug;16(4):553-60.

    41. 2009年

  41. Soma T, Nishida K, Yamato M, Kosaka S, Yang J, Hayashi R, Sugiyama H, Maeda N, Okano T and Tano Y. Histologic evaluation of mechanical epithelial separation with an epi-LASIK procedure using the Epi-K Epikeratom. J Cataract Refract Surg. 2009 Jul;35(7):1251-9.
  42. Kanayama S, Nishida K, Yamato M, Hayashi R, Maeda N, Okano T, Tano Y. Analysis of Soluble Vascular Endothelial Growth Factor Receptor-1 Secreted from Cultured Corneal and Oral Mucosal Epithelial Cell Sheets in Vitro. Br J Ophthalmol. 2009 Feb;93(2):263-7.

    43. 2008年

  43. Sugiyama H, Maeda K, Yamato M, Hayashi R, Soma T, Hayashida Y, Yang J, Shirakabe M, Matsuyama A, Kikuchi A, Sawa Y, Okano T, Tano Y and Nishida K, Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells. J Tissue Eng Regen Med. 2008 Oct;2(7):445-9.
  44. Hayashi R, Yamato M, Saito T, Oshima T, Okano T, Tano Y, Nishida K. Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin alpha6 and CD71. Biochem Biophys Res Commun. 2008 Mar 7;367(2):256-63.

    45. 2007年

  45. Kanayama S, Nishida K, Yamato M, Hayashi R, Sugiyama H, Soma T, Maeda N, Okano T, Tano Y. Analysis of angiogenesis induced by cultured corneal and oral mucosal epithelial cell sheets in vitro. Exp Eye Res. 2007 Dec;85(6):772-81.
  46. Tsuruta H, Hayashi R, Ohkawa H, Ohkatsu N, Morimoto M, Nishimoto K, Santou S, Aizono Y. Characterisitics and gene cloning of phospholipase D of the psychrophile, Shewanella sp. Biosci Biotechnol Biochem. 2007 Oct;71(10):2534-42.
  47. Murayama K, Kimura T, Tarutani M, Tomooka M, Hayashi R, Okabe M, Nishida K, Itami S, Katayama I, Nakano T. Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors. Oncogene. 2007 Jul 19;26(33):4882-8.
  48. Hayashi R, Yamato M, Sugiyama H, Sumide T, Yang J, Okano T, Tano Y, Nishida K. N-Cadherin is expressed by putative stem/progenitor cells and melanocytes in the human limbal epithelial stem cell niche. Stem Cells. 2007 Feb;25(2):289-96.